Mice where cheek-bled one day after the trauma reminder to measure differential peripheral CRP protein expression post trauma reminder. Two weeks post predator stress, we assessed avoidance of trauma cues by measuring exploration of a cue scented with dirty cat litter.
After one week, mice were tested for avoidance behaviors by open field and light-dark box testing.
Four weeks after infection, mice were exposed to either predator stress (10 minutes roomed with a laboratory cat) or handled (stress control). Methods: Male C57BL6J mice received a single intrajugular injection of 1011 genome copies (gc) of either AAV8.CRP or AAV8.Null. We tested the hypothesis that increased CRP after gene transfer using an adeno-associated virus (AAV8) encoding murine CRP may confer a higher risk for PTSD-like behaviors after predator stress. CRP genetic mutations have also been positively correlated with increased PTSD symptoms, further suggesting CRP may confer risk for PTSD pathogenesis (Michopoulos et al., 2015). Moreover, one prospective study demonstrated that marines who were diagnosed with post-deployment PTSD previously had a two-fold higher baseline serum CRP levels prior to deployment (Eraly et al., 2014). Several large case-control and prospective studies have shown associations with PTSD diagnosis and elevated serum peripheral inflammatory markers, including the acute phase reactant C-reactive protein (CRP) (Spitzer et al., 2010 Passos et al., 2014). Increasing evidence suggests a role for systemic and neurological inflammation in the pathophysiology of fear and trauma exposure based psychiatric disorders (Micholpoulos et al., 2017 Haroon et al., 2012).
Kirk Hammond, Victoria Risbrough University of California, San Diego School of Medicine, San Diego, California, United Statesīackground: Post-traumatic stress disorder (PTSD) remains a growing and often debilitating psychiatric disorder resulting from severe trauma. Samantha Friend*, Sorana Caldwell, Rahul Nachnani, April Crossan, Mei Hua Gao, Ngai C.
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